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Image Search Results
Journal: Arthritis Research & Therapy
Article Title: The oncoprotein TBX3 is controlling severity in experimental arthritis
doi: 10.1186/s13075-018-1797-3
Figure Lengend Snippet: In vitro proliferative response of CD19 + B cells and CD4 + T cells from B10.RIIIS/J- Eae39r2 sub-congenic mice. 3 H-thymidine incorporation, represented as cpm ± SEM, of CD19 + B cells following in vitro stimulation with titrated concentrations (0–40 μg/ml) of anti-IgM antibody ( a ); titrated concentrations (0–10 μg/ml) of lipopolysaccharide LPS ( b ); combination of fixed concentration (10 ng/ml) of IL-4 and titrated concentrations (0–4 μg/ml) of anti-CD40 antibody ( c ). d 3 H-thymidine incorporation of CD4 + T cells following in vitro stimulation with combination of titrated concentrations (0–3 μg/ml) of anti-CD3 antibody and fixed concentration of anti-CD28 antibody (3 μg/ml). e IL-2 production (mean ± SEM) in the supernatant of CD4 + T cells stimulated with anti-CD3/CD28 antibodies. Statistical analyses were performed using the Mann-Whitney U test; * p < 0.05
Article Snippet: CD19 + B lymphocytes for cell proliferation assay, real time PCR, and TBX3 activity assay, were positively selected from splenic cell suspensions by using the
Techniques: In Vitro, Concentration Assay, MANN-WHITNEY
Journal: Arthritis Research & Therapy
Article Title: The oncoprotein TBX3 is controlling severity in experimental arthritis
doi: 10.1186/s13075-018-1797-3
Figure Lengend Snippet: Quantitative analysis of active TBX3 in nuclear lysates of CD19 + B cells from B10.RIII mice. a Comparison of active TBX3 levels in CD19 + B cells isolated from naïve B10.RIII mice ( n = 4), with and without in vitro stimulation with a fixed concentration (10 μg/ml) of anti-IgM antibody for 48 h. b , c Collagen-induced arthritis (CIA) development and corresponding TBX3 activity in B10.RIII mice whereby four mice were killed at indicated time points after collagen type II (CII) immunization and levels of active TBX3 were determined with and without ex vivo re-stimulation with fixed concentration (10 μg/ml) of anti-IgM antibody for 48 h. Statistical analyses were performed using the Mann-Whitney U test; *significant ( p < 0.05) compared to day 0 unstimulated samples; # significant compared to day 7 unstimulated samples; § significant compared to respective unstimulated samples
Article Snippet: CD19 + B lymphocytes for cell proliferation assay, real time PCR, and TBX3 activity assay, were positively selected from splenic cell suspensions by using the
Techniques: Comparison, Isolation, In Vitro, Concentration Assay, Activity Assay, Ex Vivo, MANN-WHITNEY